Determination of 10 mycotoxins in Hippophae Fructus medicinal and edible products by ultra-performance liquid chromatography- tandem mass spectrometry / 中国中药杂志

An analytical method for 10 mycotoxins in Hippophae Fructus medicinal and edible products was established in this study, and the contamination of their mycotoxins was analyzed. First of all, the mixed reference solution of ten mycotoxins such as aflatoxin, ochratoxin, zearalenone, and dexoynivalenol was selected as the control, and the Hippophae Fructus medicinal and edible products were prepared. Secondly, based on the ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) technology, 10 mycotoxins in Hippophae Fructus medicinal and edible products were quantitatively investigated and their content was determined. Finally, the contamination of mycotoxins was analyzed and evaluated. The optimal analysis conditions were determined, and the methodological inspection results showed that the 10 mycotoxins established a good linear relationship(r>0.99). The method had good repeatability, test sample specificity, stability, and instrument precision. The average recovery rates of 10 mycotoxins in Hippophae Fructus medicinal products, edible solids, and edible liquids were 90.31%-109.4%, 87.86%-107.8%, and 85.61%-109.1%, respectively. Relative standard deviation(RSD) values were 0.22%-10%, 0.75%-13%, and 0.84%-8.5%, repsectively. Based on UPLC-MS/MS technology, the simultaneous determination method for the limits of 10 mycotoxins established in this study has fast detection speed, less matrix interference, high sensitivity, and accurate results, which is suitable for the limit examination of 10 mycoto-xins in Hippophae Fructus medicinal and edible products.

Colombian vs. American corn: Quality, chemical composition, mycotoxin content and metabolizable energy

Background: Corn is, quantitatively, one of the most important world crops (ranking second only after wheat) and a key ingredient in animal feeds. Objective: to assess and compare corn quality, mycotoxin content, chemical composition and apparent metabolizable energy (AME) of domestic and imported corn. Methods: Grain quality (USDA grading system) was determined in 30 samples of domestic and 21 samples of imported corn. From each origin, 15 samples were subjected to proximal analysis and 10 were used to determine fatty acid composition. Mycotoxin analysis was conducted on 30 samples of domestic and 23 of imported corn. Results: six of the 30 domestic samples corresponded to US1 grade (highest quality) vs. none of the imported. In the "sample grade" category (lowest quality), 10 and 6 samples corresponded to imported and domestic corn, respectively. Soybeans were found as contaminant in 15 of the 21 imported corn samples. Aspergillus spp. mycotoxins such as ochratoxin A were not detected, and aflatoxins were found in only a few samples at very low levels. Fusariotoxins such as deoxynivalenol and zearalenone were found in 61 and 43% of imported samples, respectively, but in none of the domestic samples. Domestic corn had lower carbohydrate content compared with imported corn (85.4 vs. 86.7%), but higher crude fat (3.8 vs. 3.1%). The AME values for domestic and imported corn were 3,697 and 3,378 kcal/kg, respectively. The fatty acid profiles from both corn types were similar. Conclusion: This study found significant differences between locally-grown and imported corn, particularly in terms of crude fat, AME content, fusariotoxins, and contaminant seeds (soybeans). These findings suggest that locally-grown corn might have nutritional and toxicological advantages over corn imported from the United States.

Antecedentes: El maíz es, cuantitativamente, uno de los cultivos más importantes a nivel mundial (ocupa el segundo lugar después del trigo) y uno de los principales ingredientes en dietas para animales. Objetivo: Evaluar y comparar la calidad del maíz, su contenido de micotoxinas, composición química, y energía metabolizable aparente (AME) del maíz nacional e importado. Métodos: La calidad del grano (sistema de clasificación de la USDA) se determinó en 30 muestras de maíz producido en Colombia y 21 de maíz importado. De cada origen, 15 muestras se sometieron a análisis proximal y 10 se analizaron para determinar el perfil de ácidos grasos. El contenido de micotoxinas se determinó en 30 muestras de maíz nacional y 23 de maíz importado. Resultados: Seis de las treinta muestras nacionales analizadas para calidad de grano correspondieron a grado US1 (la mejor calidad) frente a ninguna de las importadas. En categoría "grado muestra" (la menor calidad) se encontraron 10 y 6 de las muestras de maíz importado y nacional, respectivamente; 15 de las 21 muestras de maíz importado presentaron contaminación con semillas de fríjol soya. No se encontraron micotoxinas de hongos Aspergillus spp. (ocratoxina A), y niveles muy bajos de aflatoxinas en unas pocas muestras. Fusariotoxinas tales como deoxinivalenol y zearalenona se detectaron en el 61 y 43% de las muestras de maíz importado, respectivamente, pero en ninguna muestra de maíz nacional. El maíz nacional presentó menor contenido de carbohidratos (85,4 vs. 86,7%), pero mayor contenido de grasa cruda (3,8 vs. 3,1%) en comparación con el importado, respectivamente. Los valores de AME fueron de 3.697 y 3.378 kcal/kg para el maíz nacional e importado, respectivamente. El perfil de ácidos grasos de ambas procedencias fue similar. Conclusiones: Este estudio encontró diferencias significativas entre el maíz de producción nacional y el importado, en especial en su contenido de grasa cruda y AME, fusariotoxinas, y semillas contaminates (soya). Estos hallazgos sugieren que el maíz de producción nacional puede presentar ventajas de tipo nutricional y toxicológico frente al maíz importado de los Estados Unidos de América.

Antecedentes: O milho é quantitativamente uma das culturas mais importantes em nível mundial (ocupando o segundo lugar depois do trigo), sendo um dos principais ingredientes em dietas para animais. Objetivo: Avaliar e comparar a qualidade do milho, o teor de micotoxinas, a composição química e a energia metabolizável aparente (EMA) do milho nacional e importado. Métodos: A qualidade do grão (sistema de classificação do USDA) foi determinada em 30 amostras de milho produzido na Colômbia e 21 amostras de milho importado. Quinze amostras de cada tipo de milho foram submetidas a análise proximal e dez amostras de cada milho foram analisadas para determinar o perfil de ácidos graxos. O conteúdo de micotoxinas foi determinado em 30 amostras de milho da Colômbia e em 23 amostras de milho importado. Resultados: Seis das trinta amostras colombianas analisadas para qualidade de grão corresponderam a grau US1 (melhor qualidade) frente a nenhuma das amostras importadas. Na categoria "grau amostra" (menor qualidade) foram encontradas 10 e 6 amostras de milhos importados e colombianos, respectivamente; Quinze das 21 amostras de milho importado apresentaram contaminação com sementes de feijão soja. Não foram encontradas micotoxinas de fungos Aspergillus spp. (ocratoxina A) ou apenas níveis muito baixos em poucas amostras (aflatoxinas). As fusariotoxinas, deoxinivalenol e zearalenona foram detectadas em 61 e 43% das amostras de milho importado, respectivamente, mas não foram detectadas em nenhuma amostra do milho colombiano. O milho colombiano apresentou menor conteúdo de carboidratos (85,4 vs. 86,7%) porém maior conteúdo de gordura crua (3,8 vs. 3,1%). Os valores de AME foram 3.697 e 3.378 kcal/kg para as amostras de milho colombiano e importado, respectivamente. O perfil de ácidos graxos de ambos tipos de milho foi similar. Conclusões: Os resultados do presente estudo mostram diferenças significativas entre o milho de origem colombiana e o milho importado dos Estados Unidos, em especial no conteúdo de gordura crua e AME, fusariotoxinas e sementes contaminantes (soja). Estes achados sugerem que o milho de produção colombiana pode apresentar vantagens de tipo nutricional e toxicológica frente ao milho importado dos Estados Unidos.

Application of Syzygium aromaticum, Ocimum sanctum, and Cananga odorata essential oils for management of Ochratoxin A content by Aspergillus ochraceus and Penicillium verrucosum: An in vitro assessment in maize grains

The study is directed to establish the minimizing effects of Syzygium aromaticum, Ocimum sanctum, and Cananga odorata essential oils on the growth and ochratoxin A (OTA) level of Aspergillus ochraceus and Penicillium verrucosum in maize grains. S. aromaticum essential oil (SAEO), O. sanctum essential oil (OSEO), and C. odorata essential oil (COEO) were extracted by hydro-distillation technique, and a total of 50, 44, and 48 chemical constituents were identified by gas chromatography-mass spectrometry (GC-MS), respectively.The SAEO and OSEO belong to the chemotype of eugenol, whereas, COEO was found to be the chemotype of thymol, limonene, and ?-ylangene. The antifungal activity of essential oils (EOs) was determined by the micro-well dilution technique. The SAEO showed superior antifungal activity compared to OSEO, COEO, and synthetic antifungal agent nystatin, and its minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values against A. ochraceous and P. verrucosum were noticed as 1251 ± 42.32 and 1878 ± 28.47 µg/mL, and 0815 ± 22.69 and 1146 ± 51.19 µg/mL, respectively.The antifungal mechanism of EOs was unveiled by assessing the intracellular reactive oxygen species (ROS), ergosterol content, and membrane integrity. The antifungal investigations found that EOs caused fungal mortality by increasing the intracellular ROS, depleting ergosterol synthesis, and distracting membrane integrity. Finally, antifungal and antimycotoxin activity of EOs was demonstrated in maize grains. The SAEO, OSEO, and COEO have reduced the complete fungal growth and OTA level of A. ochraceous and P. verrucosum correspondingly at 2500 and 2500, 3500 and 2500, and 3500 and 3500 µg/g in maize. The EOs could act as natural antifungal agents; protect foodstuffs from fungal infection and mycotoxins during storage.

Development of an approach for simultaneous determination ofmulti-mycotoxins in fresh fruits / 预防医学

Objective@#To develop an approach for simultaneous detection of multi-mycotoxins in fresh fruits, so as to provide technical supports for mycotoxins surveillance in fresh fruits.@*Methods@#Fresh fruits were collected from markets and homogenized. Then, 2 g of fresh fruits were added with 10 mL of 0.1% formic acid ( 99∶1, v/v ) in acetonitrile and wortexed for 10 min. Following extraction with 1 g of sodium chloride and 4 g of anhydrous sodium sulfate, samples were centrifuged and 5 mL of the supernatant was cleaned up with 25 mg C18. Following centrifugation, the supernatant was dried under nitrogen. The residue was dissolved in 300 μL of methanol-acetonitrile mixture solution ( 1∶1, v/v ), and mixed evenly in 700 μL of the distilled water. Samples were then eluted in gradient series of 0.1% formic acid and 5 mmol ammonium formate and methanol-acetonitrile mixture solution ( 1∶1, v/v ). The 15 mycotoxins were determined using liquid chromatography-tandem mass spectrometry ( LC-MS/MS ) with electrospray ion source (ESI+/ESI-) under multiple reaction monitoring. In addition, a matrix-matched standard curve was employed for quantitative analysis.@*Results@#There was a good linear relationship for 15 mycotoxins at concentrations of 0.25 to 10 ng/mL ( R2>0.992 ), the LC-MS/MS method showed the detection limits of 0.1-1.0 μg/kg, the spiked recovery rates of 71.68%-117.50%, and the relative standard deviations ( RSDs ) of 0.01%-13.60%. The detection rate of mycotoxins was 27.09% in 203 fresh fruits sold in markets.@*Conclusions@#The optimized LC-MS/MS method can be used for simultaneous determination of multi-mycotoxins in fresh fruits.

Micotoxinas en el análisis de los alimentos: Un estudio con indicadores bibliométricos y mapas de visualización / Mycotoxins in food analysis: A study with bibliometric indicators and visualization maps

El presente estudio tuvo como objetivo identificar el desarrollo de la producción científica según año y revistas; determinar la estructura temática e identificar la red de coautoría de la literatura sobre micotoxinas en el análisis de los alimentos. Se realizó un estudio descriptivo con indicadores bibliométricos y mapas de visualización, en el que se usó la base de datos Scopus de los documentos incluidos desde el año 2011 al 2020. Se consideraron 453 artículos que cumplieron con los criterios de inclusión, 382 artículos de investigación y 71 revisiones. El promedio de citas por artículo varió anualmente entre 6 (2019) y 40,5 (2012). La revista más productiva fue Mycotoxin Research, de la editorial Springer Nature. En el mapa de coocurrencias se obtuvieron 5 clústeres de descriptores. En el mapa de coautoría se hallaron 21 clústeres, 14 de ellos correspondieron a autores que investigaron en coautoría. El desarrollo científico tuvo una producción anual no uniforme, con crecimiento lento. La estructura temática según la red de coocurrencia indica que el término más frecuente es micotoxinas, correspondiente al enfoque micotoxinas que influyen en la salud pública del primer clúster. La red de coautoría indica que el 98,2 por ciento de los artículos fueron elaborados en coautoría y el autor más productivo fue Krska Rudolf, de la Queen's University Belfast, con 24 artículos(AU)

The purpose of the study was to identify the development of scientific production by year and journal, determine the thematic structure and identify the co-authorship network of the literature about mycotoxins in food analysis. A descriptive study was conducted of documents published in the database Scopus from 2011 to 2020, using bibliometric indicators and visualization maps. A total 453 articles were included which met the inclusion criteria. Of these, 382 were research articles and 71 were reviews. The average annual number of citations per article ranged from 6 in 2019 to 40.5 in 2012. The most productive journal was Mycotoxin Research from Springer Nature publishers. The co-occurrence map displayed five descriptor clusters, whereas the co-authorship map displayed 21 clusters, 14 of which corresponded to authors researching in co-authorship. Scientific development had a non-uniform, slowly growing annual output. The thematic structure according to the co-occurrence network shows that the most frequent term is mycotoxins, corresponding to mycotoxins influencing public health from the first cluster. The co-authorship network shows that 98.2percent of the articles were co-authored, and the most productive author was Krska Rudolf, from Queen's University, Belfast, with 24 articles(AU)

Efeitos antifúngico e antiaflatoxigênico de timol e carvacrol em Aspergillus flavus / Antifungal and antiaflatoxigenic activities of thymol and carvacrol against Aspergillus flavus

O objetivo deste estudo foi avaliar os efeitos do timol e carvacrol contra Aspergillus flavus e a produção de aflatoxinas. Foram determinadas a concentração inibitória mínima (CIM), o espectro fungitóxico e a atividade inibidora de micotoxinas pelo timol e carvacrol. Os resultados mostraram que timol e carvacrol exibiram ação fungicida de acordo com a CIM de 2500 e 30 µg mL-1, respectivamente. Tanto o timol quanto o carvacrol inibiram significativamente o crescimento de A. flavus (p<0,05) a partir de 600 e 15 µg mL-1, respectivamente. A biomassa fúngica, estimada pela determinação da concentração de ergosterol, foi significativamente reduzida (p <0,05) em 2500 µg mL-1 de timol e 250 µg mL-1 de carvacrol. O timol e o carvacrol exibiram efeitos antiaflatoxigênicos em 600 e 125 µg mL-1, respectivamente. O timol e o carvacrol exibiram atividade antifúngica, mas não exibiram alta atividade antiaflatoxigênica. Carvacrol e timol podem ser considerados como potentes compostos naturais antifúngicos contra A. flavus.

The aim of this study was to evaluate the effects of thymol and carvacrol upon production of aflatoxins by Aspergillus flavus and upon its growth. Minimal inhibitory concentration (MIC), fungitoxic spectrum and mycotoxin inhibition were assessed. Results showed thymol and carvacrol exhibited fungicidal action, as determined by MIC values of 2500 and 30 µg mL-1, respectively. Both thymol and carvacrol significantly inhibited growth of A. flavus (p<0.05) at concentrations of 600 and 15 µg mL-1, respectively. Fungal biomass, as estimated by determination of ergosterol concentration, was significantly reduced (p<0.05) at thymol concentrations of 2500 µg mL-1 and at carvacrol concentrations of 250 µg mL-1. Thymol and carvacrol exhibited antiaflatoxigenic effects at concentrations of 600 and 125 µg mL-1, respectively. While both thymol and carvacrol showed possessing antifungal activities, neither were highly antiaflatoxigenic. Carvacrol and thymol might be considered for use as potential antifungal natural compounds against A. flavus.

Toxigenic fungal species and natural occurrence of mycotoxins in crops harvested in Argentina / Especies fúngicas toxigénicas y ocurrencia natural de micotoxinas en cultivos y productos cosechados en Argentina

Abstract Mycotoxins are secondary metabolites produced by fungal species that mainly belong to Aspergillus, Fusarium, Penicillium and Alternaria, which can grow in a variety of crops including cereals, oilseeds and fruits. Consequently, their prevalence in foods and by-products not only affects human and animal health but also causes important losses in both domestic and international markets. This review provides data about toxigenic fungal species and mycotoxin occurrence in different crops commonly grown in Argentina. This information will be relevant to establish adequate management strategies to reduce the impact of mycotoxins on human food and animal feed chains and to implement future legislation on the maximum permitted levels of these fungal metabolites.

Resumen Las micotoxinas son metabolitos secundarios producidos por diferentes especies fúngicas pertenecientes, principalmente, a los géneros Aspergillus, Fusarium, Penicillium y Alternaria. Dichos microorganismos pueden crecer en una gran variedad de cultivos, entre los que se incluyen cereales, oleaginosas y frutas. La presencia de micotoxinas en alimentos y subproductos no sólo afecta la salud humana y animal, sino que también causa pérdidas importantes en los mercados nacionales e internacionales. Esta revisión proporciona datos sobre la prevalencia de especies fúngicas toxigénicas y de micotoxinas en diferentes cultivos y productos cosechados en Argentina. Dicha información será relevante para establecer estrategias de manejo adecuadas para reducir la entrada de las micotoxinas en las cadenas alimentarias del hombre y de los animales, así como para establecer futuras legislaciones sobre los niveles máximos permitidos de dichos metabolitos.

Horses fed meal contaminated with mycotoxins performing short-term exercises / Efeitos das micotoxinas na performance de equinos submetidos a exercícios de curta duração

This research was carried out to evaluate the effect of mycotoxins on the performance of horses through physiological parameters, and hematology and serum biochemistry analyses. The essay lasted 40 days, with 12 days for adaptation and 28 days of experimentation. In the experimental stage, the horses were distributed in a completely randomized design, with three treatments with four animals each. The treatments used were 0 (control), 50 ppb and 100 ppb of Aflatoxin B1 (AFB1) added to a concentrate in a basal diet. The basal diet contained mycotoxins from feedstuffs naturally contaminated. The exercise test was performed over the 21th day of the experimental stage. The exercise consisted in an interval training test with a warm-up of 17 mins at a trot followed by three gallops of 450m/min. The heart rate was monitored between the gallops. Before the exercise test and immediately after the third gallop, the physiological and blood parameters were evaluated, and continued up to 48 hours after the exercise. The results of the physiological, hematological and biochemical parameters were submitted to analysis of variance (ANOVA) and compared by the Tukey test at 5% of significance. The presence of AFB1 in the diet influenced the alkaline phosphatase activity, which presented higher values in horses fed diet with inclusion of 100 ppb AFB1, suggesting a hepatotoxic activity associated with the others mycotoxins naturally present in the feedstuffs.(AU)

Esta pesquisa foi conduzida para avaliar o efeito de micotoxinas no desempenho de equinos com avaliações fisiológicas e análises hematológicas e da bioquímica sérica. O ensaio durou 40 dias, com 12 dias de adaptação e 28 dias de experimentação. Na fase experimental, os equinos foram distribuídos em delineamento inteiramente casualizado em três tratamentos, com quatro animais cada. Os tratamentos utilizados foram 0 (controle), 50 ppb e 100 ppb de Aflatoxina B1 (AFB1) adicionada ao concentrado de uma dieta basal. A dieta basal continha alimentos naturalmente contaminados por micotoxinas. O teste de desempenho foi executado no 21º dia da fase experimental por meio de teste intervalado consistindo em aquecimento ao trote por 17 minutos, seguido de três galopes de 450m/min. A frequência cardíaca (FC) foi monitorada entre os galopes. Antes do exercício e imediatamente após o terceiro galope, os parâmetros fisiológicos e sanguíneos foram avaliados e continuaram sendo monitorados até 48 horas após o exercício. Os resultados dos parâmetros fisiológicos, hematológicos e bioquímicos foram submetidos à análise de variância (ANOVA) e comparados pelo teste de Tukey a 5% de significância. A presença de AFB1 na dieta influenciou a atividade da fosfatase alcalina, que apresentou valores mais elevadas na dieta com inclusão de 100 ppb de AFB1, sugerindo uma atividade hepatotóxica associada às outras micotoxinas naturalmente presentes nos alimentos.(AU)

Simultaneous determination of 15 mycotoxins in peanuts by ultra high performance liquid chromatography-tandem mass spectrometry with QuEChERS EMR-Lipid approach and stable isotope dilution / 上海预防医学

Objective To develop a method for the simultaneous determination of 15mycotoxins in peanuts by ultra high performance liquid chromatography-tandem mass spectrometry with QuEChERS EMR-Lipid approach and stable isotope dilution. Methods The samples were extracted by 2% formic acid acetonitrile-water (50 : 50, V/V) and then purified with QuEChERS EMR-Lipid approach.The mycotoxins were fully separated on a pentafluorophenyl column under a gradient elution with methonal-0.01%formic acid aqueous solution.The mycotoxins were analyzed by UPLC-MS/MS with multiple reaction monitoring (MRM) mode and quantified by isotope internal standard method. Results Fifteen mycotoxins had good linear relationship in the certain correlation ranges with the correlation coefficients all above 0.995 and the detection limits were 0.1－10 μg/kg.The mean recoveries ranged from 81.2% to 115.3% with RSD (n=6) varying from 2.1% to 10.7%. Conclusion The method is simple, highly sensitive, practical, and proves to be suitable for quantitative analysis of 15 mycotoxins in peanuts.

Micotoxinas e seus efeitos à saúde humana: revisão de literatura / Mycotoxins and their effects on human health: literature review

Fungos são microrganismos que se desenvolvem em ambientes úmidos, produzindo, como metabolito secundário, micotoxinas, que contaminam alimentos e trazem danos à saúde. As micotoxinas são metabólicos produzidos por determinados gêneros de fungos filamentosos que acometem os produtos alimentícios e que, quando ingeridos, podem causar distúrbios ao organismo humano. Fungos se desenvolvem nos alimentos em qualquer fase do processo tendo condições favoráveis a eles, e uma vez que o alimento seja acometido pelo fungo as micotoxinas podem permanecer no alimento após a morte do fungo que as produz. Logo se faz pensar nos produtos ofertados. Este estudo constitui-se de uma revisão da literatura especializada, enfatizando os danos que os alimentos com micotoxinas podem causar ao organismo do ser humano. Para a coleta utilizaram-se o banco de dados Medline e os sites National Libray of Medicine (PubMed) e Scientific Eletronic Libray Online (SciELO), por meio das palavras-chave: fungos filamentosos; micotoxinas; produtos alimentícios. Sabe se que há um vasto número de micotoxinas, mas visando não só a perda econômica, mas também a saúde, pesquisas vêm se concentrando em algumas micotoxinas por apresentarem danos significativos já comprovados à saúde humana e animal, sem se descartar a possibilidade de outras apresentarem risco a saúde humana.

Fungi are microorganisms that grow in humid environments, producing, as a secondary metabolite, mycotoxins that contaminate food and bring harm to health. Metabolic mycotoxins are produced by certain genera of filamentous fungi that affect food products and which when ingested can cause disturbances to the human organism. Fungi develop in foods at any stage of the process having favorable conditions for them, and once the food is affected by the fungus the mycotoxins may remain in the food after the death of the fungus that produces them. One soon thinks about the products offered. This study is a review of the specialized literature, emphasizing the damage that foods with mycotoxins can cause to the human body. For collection it was used the Medline database and the National Libray of Medicine (Pubmed) and Scientific Electronic Libray Online (SciELO) websites, through the keywords: filamentous fungi; mycotoxins; food products. It is known that there is a large number of mycotoxins, but aiming not only economic loss, but also health, research has focused on some mycotoxins due to already proven significant damage to human and animal health, without ruling out the possibility of others presenting risk to human health.

Mycotoxin Contamination Risks Due to Handling Practices of Indigenous Chicken Feeds in Kenya

Aim: Mycotoxins are prevalent in animal feeds and agricultural products. These toxins are produced by fungi and once incorporated in the substrate, are not easy to eradicate. They are associated with morbidity and mortality in both livestock and humans. Avoiding contamination is the preferred way of mitigating mycotoxins in livestock feeds and cereals. Study Design: A purposive multiple-stage survey design was used in this study. Place and Duration of Study: Between February and March 2016 to assess factors that exacerbate mycotoxins due to feed type and handling practice by smallholder farmers in farmer groups keeping indigenous chicken in Western Kenya. Methodology: Three counties Siaya, Busia and Kakamega of Kenya were selected based on the population of indigenous chicken. Semi-structured questionnaires were used in gathering data on feed types, handling practices and mycotoxins awareness from 180 farmers in women and youth groups. Results: Common feed types identified included maize (96%), sorghum (54%), cassava (42%), millet (40%), homemade rations (16%), while 44% used commercial feeds. It was noted that 38% use rotten, insect-infested, unsorted and broken cereals and 62% clean cereals as feeds. For storage, 85% and 7% of farmers were using polypropylene and hermetic bags, respectively; 97% dried their grains/feeds on a platform; 21% were not sorting their grains and 17% were not using grain preservatives during storage. Mycotoxin awareness levels were assessed among farmers. Approximately 44% of farmers were unaware of feed safety while 71% were aware of mycotoxins; however, 73% of participants were unaware of dangers posed by mycotoxin contamination in feeds. Conclusion: Information to farmers on mycotoxin and proper feed and cereals handling and storage practices is necessary for mycotoxin management. Therefore, avoiding contamination is a preferred method of mitigating mycotoxins in indigenous chicken feeds and cereals.

The sanitary quality of Tifton 85 bermudagrass and Wrangler grass hay stored under different environmental conditions / Variações da temperatura e qualidade sanitária de f eno de capim tifton 85 e vaquero grass sob diferentes condições de armazenament

This study evaluated the variations in temperature and dry matter content in Tifton 85 bermudagrass and Wrangler grass hay stored under different environmental conditions. The fungal and mycotoxin populations in the hay production area were also evaluated. Fifty samples (30 hay bales, ten samples of organic matter [OM], and ten samples of grass [G] before baling) were used in a completely randomized design and factorial scheme with ten treatments (a combination of two classes of G: Tifton 85 bermudagrass and Wrangler grass) and three storage systems: a covered shed without side walls, covered with a clear tarp; an open environment covered with a double-sided tarp; and a ventilated closed shed. Four additional treatments, five replications, and one sample per experimental unit completed the study design. The environment influenced the temperature of the Tifton 85 bermudagrass and Wrangler grass bales, and the dry matter content of the bales during the evaluated period was above the recommended limit (800 g kg-1). The genus Penicillium occurred with the highest frequency in the Wrangler grass stored under a double-sided tarp (6.13 log CFU g-1). In the additional treatment, the OM deposited in the soil of the Wrangler grass production area exhibited a greater occurrence of the fungus Fusarium (4.66 log CFU g-1) and of the mycotoxin zearalenone (ZEA; 594.2 µg kg-1). The hay stored in the shed with a clear tarp exhibited more aflatoxin (AFLA) and deoxynivalenol (DON). The concentration of mycotoxins varied across the storage environments and between the stored species; however, the shed storage system used in hay production might also be a source of hay contamination via the incorporation of decomposing OM with mycotoxins in the hay.

Este estudo teve como objetivo avaliar as variações na temperatura e no teor de matéria seca do feno de capim Tifton 85 e Vaquero armazenados em diferentes condições ambientais. As populações de fungos e micotoxinas na área de produção de feno também foram avaliadas. Cinquenta amostras (30 fardos de feno, dez amostras de matéria orgânica [OM] e dez amostras de gramíneas [G] antes do enfardamento) foram utilizadas em delineamento inteiramente casualizado e esquema fatorial com dez tratamentos (uma combinação de duas classes de G: Tifton 85 e capim Vaquero) e três sistemas de armazenamento: galpão coberto sem paredes laterais, feno coberto com uma lona transparente; ambiente aberto e o feno coberto com uma lona dupla face; e galpão fechado ventilado. Quatro tratamentos adicionais, cinco repetições e uma amostra por unidade experimental completaram o desenho experimental. O ambiente influenciou a temperatura dos fardos de Tifton 85 e de capim vaquero, e o teor de matéria seca dos fardos durante o período avaliado foi acima do limite recomendado (800 g kg-1). O gênero Penicillium ocorreu com maior freqüência no capim Vaquero armazenado sob uma lona de dupla face (6,13 log CFU g-1). No tratamento adicional, a MO depositada no soloda área de produção de capim Vaquero apresentou maior ocorrência do fungo Fusarium (4,66 log CFU g-1) e da micotoxina zearalenona (ZEA; 594,2 µg kg-1). O feno armazenado no galpão e coberto com lona transparente apresentou maiores teores de aflatoxina (AFLA) e desoxinivalenol (DON). A concentração de micotoxinas variou entre os ambientes de armazenamento e entre as espécies armazenadas; no entanto, a contaminação do feno pode se dar pela incorporação de MO com micotoxinas presentes no campo.

Neurological diseases in cattle caused by plants and mycotoxins in Santa Catarina state, Brazil / Enfermidades neurológicas em bovinos causadas por plantas e micotoxinas no estado de Santa Catarina

This study described the epidemiological and clinical-pathological aspects of 25 outbreaks of neurological diseases in cattle caused by plants and mycotoxins in Santa Catarina state. Six of them were due to Sida carpinifolia poisoning, five to Solanum fastigiatum, five to Phalaris angusta, three to Claviceps paspali, three to Claviceps purpurea, and three outbreaks were of unknown etiology. The clinical signs observed in the affected cattle were mild to severe and characterized by generalized muscle tremors, incoordination, hypermetria, wide-based stance, intentional head tremors, dull staring eyes, and frequent ear twitching, with convulsions in some cases. At necropsy, lesions were observed only for P. angusta poisoning, characterized by gray-greenish discoloration in thalamus and midbrain. Microscopically, rarefaction and/or disappearance of Purkinje neurons with substitution by Bergmann cells were observed for S. carpinifolia and S. fastigiatum poisoning. For P. angusta poisoning, thin granular brown-yellowish pigment was observed in the cytoplasm of some neurons. Gross and microscopic findings were not observed in three outbreaks of tremorgenic disease of unknown etiology. Experiments conducted with leaves, flowers and seeds of Ipomoea indivisa and Ipomoea triloba, as well as with maize and soybean residues contaminated with Ipomoea spp. did not reproduced clinical signs.(AU)

Descrevem-se os aspectos epidemiológicos e clinico-patológicos de 25 surtos de enfermidade neurológica em bovinos no estado de Santa Catarina causadas por plantas e micotoxinas. Destes, seis corresponderam a intoxicação por Sida carpinifolia, cinco por Solanum fastigiatum, cinco por Phalaris angusta, três por Claviceps paspali, três por Claviceps purpurea e três surtos de etiologia não definida. Os sinais clínicos observados nos bovinos afetados eram de intensidade leve a acentuada e caracterizados por tremores musculares generalizados, incoordenação, hipermetria, aumento da base de sustentação, balanço contínuo de cabeça, olhar atento e movimentos frequentes de orelhas, e em alguns surtos convulsões. Por meio de necropsia foram observadas alterações somente na intoxicação por P. angusta as quais caracterizaram por coloração cinza-esverdeada no tálamo e mesencéfalo. Na histologia, rarefação e/ou desaparecimento de neurônios de Purkinje com substituição por células de Bergmann foram observadas na intoxicação por S. carpinifolia e S. fastigiatum. Na intoxicação por P. angusta foi observado no citoplasma de alguns neurônios do tronco encefálico com pigmentação finamente granular marrom-amarelada. Nos três surtos de enfermidade tremorgênica com etiologia não definida não foram observadas lesões macroscópicas e microscópicas. Experimentos com folhas, flores e sementes de Ipomoea indivisa e Ipomoea triloba e resíduos de milho e soja contaminados com sementes destas duas plantas não produziram alterações clínicas.(AU)

Minerais séricos, características morfométricas ósseas e deposição de minerais ósseos de frangos de corte alimentados com dieta com inclusão de bentonita / Serum minerals, bone morphometric characteristics and deposition of bone minerals from broilers fed diet containing bentonite

O objetivo deste trabalho foi avaliar a concentração sérica de cálcio, cloretos, ferro, fósforo e magnésio, as características morfométricas ósseas e a deposição de cálcio e fósforo nas tíbias de frangos de corte recebendo dieta com zero, 0,25 ou 0,50% de bentonita. Um ensaio foi conduzido com 288 frangos de corte de 14 a 21 dias de idade, submetidos a três dietas experimentais: sem inclusão (0,0); com inclusão de 0,25 e com inclusão de 0,50% do adsorvente bentonita. Não foram observadas diferenças (P>0,05) no desempenho das aves, nos níveis séricos de cálcio, cloretos, ferro e magnésio, no entanto os níveis de fósforo foram reduzidos (P<0,05) nas aves que ingeriram dieta com 0,50% de bentonita. Em relação às tíbias, observou-se redução (P<0,05) na matéria mineral (g e %) e no teor de cálcio com a inclusão de 0,50% de bentonita. Houve redução (P<0,05) nos níveis de fósforo das tíbias com a inclusão de 0,25 e 0,50% de bentonita. Conclui-se que a inclusão de até 0,50% do adsorvente de micotoxinas bentonita na dieta de frangos de corte não altera o desempenho zootécnico das aves. A inclusão de 0,25% de bentonita, na dieta de frangos de corte, não altera a concentração dos minerais séricos e a deposição de minerais nas tíbias, entretanto a inclusão de 0,5% reduz os níveis séricos de fósforo, o teor de matéria mineral e a concentração de cálcio e fósforo ósseos, sem afetar as características morfométricas ósseas.(AU)

The aim of this study was to evaluate performance, serum concentration of calcium, chloride, iron, magnesium, phosphorus, and bone characteristics, ash, calcium, and phosphorus in tibias of broilers receiving diet with zero, 0.25 or 0.50% of bentonite. No differences were found on performance of poultry, on serum mineral calcium, chloride, iron, magnesium, however phosphorus levels of broilers fed on diets containing 0.5% bentonite was reduced. With respect to tibia, reduction was observed on mineral matter (g and %) and calcium levels with inclusion of 0.50% bentonite, and reduction on phosphorus levels with inclusion of 0.25 or 0.50% of bentonite on diet. We conclude that the inclusion of up to 0.50% of mycotoxin adsorbent bentonite in diet of broiler does not change broiler performance. The inclusion of 0.25% of bentonite in diet of broiler does not change serum mineral concentration and mineral deposition; however, the inclusion of 0.5% decrease serum levels of phosphorus, the content of bone mineral matter, with not effects on bone morphometric characteristics.(AU)

Mycotoxin Contamination of Food and Associated Health Risk in Cameroon: A 25-years Review (1993-2018)

The Gross Domestic Product of Cameron is partially based on agricultural products. Crops like maize, peanuts, beans, cassava, cocoa and coffee are the most cultivated ones. A high portion of this production is locally consumed, and the other part is exported to foreign countries in order to balance the economy. Postharvest losses due to fungal contamination and the presence of mycotoxins in food represent some of the most important problems this producing country government and populations are facing. The analysis of food from animal or vegatal origin in this country during the last 25 years have highlighted the presence of mycotoxins such as aflatoxins, fumonisins, ochratoxins, zearalenone and deoxynivalenol in some cases. This paper reviews the effects of mycotoxins on human health and associated regulations, their occurrence in food commodities from Cameroon, as well as the dietary exposure of consuming populations and the results obtained from their bio-monitoring.

Physiological quality and aflatoxins prevalence in conventional and transgenic soybeans during storage / Qualidade fisiológica e prevalência de aflatoxinas em sementes de soja convencional e transgênica durante o armazenamento

Physiological quality of soybean seeds of five varieties (CD 215, CD 202, CD 233RR (GMO), NK and NK 412113 7059RR (GMO)), stored under cooling and room temperature during eight months as well as B1, B2, and G2 aflatoxins prevalence were evaluated. The tests (moisture content, germination, tetrazolium) were performed before storage and every 60 days. The analysis of aflatoxins presence was carried out at the beginning and at each two months. Two samples of each variety were stored at -4 °C until analysis processing, then high-performance liquid chromatography analysis was used. The seeds showed, in general, water content between 11 and 13% until 60 storage days, whose decrease began up from 120 days. At 60 storage days, the cooled seeds had higher germination percentage when compared to the ones kept under room temperature. In relation to the studied varieties, the highest germination was recorded for CD 215, which differed from CD 202 in both periods. There were factors interactions after 120 and 240 storage days, especially under cooling to 120 days for CD 215 variety. While, at 240 days under room temperature wasobserved the highest germination for the CD 233 RR. The storage conditions were significant at 60, 180 and 240 days for viability, with a higher rate for seeds stored under cooling. Regarding varieties, over the same periods, the CD 202 variety showed lower viability percentage, with NK 412 113 after 180 and 240 days. In general, 90 samples were tested and, 25 were detected the aflatoxins presence. It was observed that the aflatoxins presence in CD 202 was quantitatively greater from 16 tested samples 10 showed some level of aflatoxin contamination. AFB1 and AFB2 aflatoxins represented 50%, while AFG2 was observed in 63% of those samples. There was no difference in relation to seed vigor between conventional or transgenic soybeans seeds. Cooling kept the vigor of CD 215 and CD 233RR varieties until the final of the experiment. There was no influence by the storage to get aflatoxins and temperature association.

Aqualidade fisiológica de sementes de soja de cinco cultivares (CD 215, CD 202, CD 233RR (GMO), NK e NK 412113 7059RR (GMO)), armazenadas sob resfriamento e temperatura ambiente durante oito meses, bem como a prevalência de aflatoxinas B1, B2 e G2 foram avaliadas. Os testes (teor de água, germinação, tetrazólio) foram antes do armazenamento e a cada 60 dias, assim como a presença de aflatoxinas. Duas amostras de cada cultivar foram armazenadas a -4 °C até o momento da análise em cromatografia líquida. As sementes mostraram, em geral, teor de água entre 11 e 13% nos 60 dias de armazenagem, decrescendo aos 120 dias. Aos 60 dias, as sementes resfriadas apresentaram alta germinação quando comparadas àquelas em temperatura ambiente. Quanto às cultivares, maior germinação foi verificada na CD 215, a qual diferiu da CD 202 em ambos os períodos. Houve interação entre os fatores depois de 120 e 240 dias de armazenagem para a CD 215. Enquanto, aos 240 dias em temperatura ambiente a maior germinação foi para a CD 233 RR. As condições de armazenamento foram significativas aos 60, 180 e 240 dias para viabilidade, com maior taxa verificada sob resfriamento. Quanto às cultivares, nos mesmos períodos, a CD 202 mostrou viabilidade mais baixa, junto da NK 412 113 depois de 180 e 240 dias. Foram testadas 90 amostras e em 25 foi detectada a presença de aflatoxinas. Na cultivar CD 202 a quantidade foi maior. De 16 amostras testadas, dez apresentaram algum nível de contaminação por aflatoxina. As aflatoxinas AFB1 e AFB2 representaram 50%, e na AFG2 foi observada em 63% das amostras. Não houve diferença quanto ao vigor entre sementes convencionais ou transgênicas. O resfriamento manteve o vigor das CD 215 e CD 233RR até o final. Em geral, não foi associada maior presença de aflatoxinas às condições de armazenamento testadas.

Identification of metabolites of Fusarium poae strain isolated from self-produced grain in a new-onset patient with Kaschin-Beck disease / 中华地方病学杂志

Objective To detect the metabolites of Fusarium poae strain isolated from self-produced grain in a new-onset patient with Kaschin-Beck disease,and to provide a scientific basis for studying the relationship between mycotoxins and Kaschin-Beck disease.Methods The strain of Fusarium poae was isolated from the self-produced spike wheat in a family of patient with new-onset Kaschin-Beck disease in Guide County,Qinghai Province in 2016.The isolated Fusarium poae strain was inoculated into potato dextrose agar (PDA) medium for enrichment culture.The metabolites of the fermentation were separated and purified,and the mycotoxins in the metabolite were detected by liquid chromatography-mass spectrometry (LC-MS).The full-spectrum identification was carried out by using the network database Metlin.Results The metabolites of this strain were identified by LC-MS and Metlin full spectrum,and mycotoxins such as trichothecenes,Fumonisin and Zearalenone (ZEA) were detected.A total of 1 601 material characteristic peaks were detected in positive ion mode,the most abundant mycotoxins were Fumonisin FP3,A2,FP1;and a total of 1 000 material characteristic peaks were detected in the negative ion mode,the most abundant were ZEA and monoacetoxyscirpenol.Conclusion The isolated metabolites of Fusarium poae strain mainly contain the three major classes of mycotoxins including trichothecenes,Fumonisins and ZEA.

Aflatoxins and fumonisins in feed from a broiler operation system from São Paulo state, Brazil / Aflatoxinas e fumonisinas em rações de um sistema de produção de frangos de corte no estado de São Paulo, Brasil

ABSTRACT: The aim of the present study was to assess the occurrence of aflatoxins (AFs) and fumonisins (FBs) in feed ingredients (corn and soybean meal) and finishing feed in a broiler operation system, as well was to evaluate their effect on the productivity of 20 batches of broilers produced and the histology status of broilers' liver after slaughter. Corn samples presented the highest frequencies of AFs and FBs, at mean levels of 29.1 and 2,100µg/kg, respectively. Soybean samples presented mean levels of 1.5 and 70µg/kg for AFs and FBs, respectively. Batches of broilers receiving feed containing FB levels higher than 1,000µg/kg had lower weight gain and higher mortality rates, while those fed rations with AFs equal or above the limit of quantification (LOQ) of the analytical method presented higher scores of histological changes in the liver. A dilution effect was observed for AFs and FBs from ingredients, especially corn, to feed during manufacture, whilst not enough to prevent losses in productivity. Results of this trial highlighted the need for strict control of mycotoxins in corn intended for broilers.

RESUMO: O objetivo do presente trabalho foi verificar a ocorrência de aflatoxinas (AFs) e fumonisinas (FBs) em ingredientes (milho e farelo de soja) e na ração de abate sobre a produtividade de uma empresa integradora de frangos de corte, bem como avaliar seus efeitos sobre produtividade de 20 lotes de frangos produzidos pela empresa e a histologia dos fígados dos frangos após o abate. As amostras de milho apresentaram as maiores frequências de AFs e FBs, em concentrações médias de 29,1 e 2.100µg/kg, respectivamente. As amostras de farelo de soja apresentaram níveis médios de 1,5 e 70µg/kg para AFs e FBs, respectivamente. Os lotes de aves que receberam ração contendo níveis de FBs maiores que 1,000µg/kg apresentaram menor ganho de peso e maior percentual de mortalidade, enquanto que as que receberam ração com AFs iguais ou superiores ao limite de quantificação (LQ) do método analítico apresentaram maior grau de alteração histopatológica no fígado. Houve efeito de diluição de AFs e FBs dos ingredientes, especialmente o milho, à ração no processo de fabricação, porém não suficiente para evitar perdas na produtividade. Os resultados do estudo reforçam a necessidade do controle estrito de micotoxinas no milho destinado à alimentação de frangos de corte.

Journey from food toxins to food safety: Transition over a century in service of nation

Since the first report of lathyrism in 1926, the ICMR-National Institute of Nutrition (NIN) at Hyderabad, India, has made tremendous contributions in the field of food toxins/food safety for the benefit of the people. The present article highlights the Institute's work on various food toxins/foodborne diseases since its inception and discusses the important contributions made in the context of public health protection that formed the basis for several national policies on their prevention and control. The investigations on food toxins, in the initial decades, were limited to the description of lathyrism and its endemicity. Subsequently, the horizon was broadened to include the problem of mycotoxins and mycotoxicosis, which had received global attention and variety of other disease outbreaks investigations leading to total food safety transition in the forthcoming decades. Important contributions in epidemiological investigations, reproduction of the disease in experimental animals, surveillance and monitoring studies, development of methods for detection of food toxins and contaminants, evolving strategies for prevention and control and developing the concept of risk assessment and risk management for addressing food safety issues in the country are discussed.

Comparative Study between Powder and Nanoparticles of Dried Cactus (Opuntia ficus-indica L.) Fruit Peels in Streptozotocin-induced Diabetic Rats: Anti-microbial and Anti-genotoxic Capacity

Aim: To overcome the toxic effects attributed to the use medicinal treatments against diabetes there is a desire toward using natural food and folk remedies. So, the aim this study was to use nanoparticles of dried cactus fruit peels (Opuntia ficus-indica) compared with powder materials to control blood glucose in streptozotocin (STZ)-induced diabetic rats. Place and Duration of Study: Food Toxicology and Contaminants Department, Polymer and Pigments Department, and Cell Biology Department, National Research Centre, Egypt, between April 2017 and March 2018. Methodology: Powder and nanoparticles were used to determine the fatty acids content and assessment the dietary fiber contents, mycotoxin contamination as well as examine the antimicrobial activity. Moreover, male albino rats were treated with single i.p. dose of STZ to induce diabetes. STZ induced-rats were divided into several groups and treated daily with 50, 100 and 200 mg/kg b.wt of cactus fruit peels powder or nanoparticles orally for 2 months. At the end of the experimental period, blood samples were aspirated to determine glucose levels as well as liver and pancreas tissues were collected for the biological analyses. Results: The results of the present study exhibited that both extracts of cactus fruit peels either powder or nanoparticles were able to reduce significantly the glucose levels and increase the expression of insulin and insulin receptor genes in induced-diabetic rats. Moreover, cactus fruit peels extracts exhibited antifungal and antibacterial activities and increase in the antioxidant enzymes (GPx and CAT) as well as anti-genotoxic effects in DM-induced rats. Furthermore, nanoparticles of dried cactus fruit peels were more effective in control glucose levels, gene expression, antimicrobial and anti-genotoxic activities compared with powder materials even in its low dose. Conclusion: The results conclude that the nanoparticles form of cactus fruit peels extracts was much more effective in the therapeutic action than powder form. The anti-diabetic effect of cactus fruit peels nanoparticles could be attributed to its content from dietary fiber. Moreover, the antifungal and antibacterial activities as well as the anti-genotoxic ability of cactus fruit peels nanoparticles could be attributed to fatty acids and/or GABA contents which were more able to control oxidative stress.